Charles University in Prague (CUNI)

This page details the publications produced by XXX that are relevant to EDENext and an overview of the key persons involved.

Petr Volf (Head of laboratory): Medical entomologist with a long-term experience in application of biochemical, immunological and histological methods in the study of pathogen-vector-host relationships. Jan Votypka (Assistant professor): medical entomologist and protozoologist, specialized in molecular phylogenetics and molecular taxonomy, with experiences in the field work on bloodsucking diptera in different countries in Europe, Middle East and Africa. Jovana Sadlova (Senior researcher): parasitologist and zoologist with outstanding skills in experimental infections of sand flies and mice by Leishmania. Iva Rohousova (Senior researcher): parasitologist specialized in immunomodulatory effects of sand fly saliva and in immune response of experimental animals and humans to sand fly bites. Vit Dvorak (Post-doc researcher) specialized in molecular taxonomy of sand flies. Vera Volfova (Researcher): biologist; skills in salivary antigens and enzymes of bloodsucking insects. Michaela Vlkova (PhD student): involved in studies on antibody response to sand fly saliva. Veronika Seblova (PhD student): involved in experimental infections of sand flies and in the fieldwork.


Badalova,J., Svobodova,M., Havelkova,H., Vladimirov,V., Vojtiskova,J., Krulova,M., Blazkova,H., Volf,P., Demant,P. & Lipoldova,M. (2004) Immunogenetics of Leishmania major infection in mice: Multiple loci and heterogeneity of gene effects. Tissue Antigens, 64, 344.

Boulanger,N., Lowenberger,C., Volf,P., Ursic,R., Sigutova,L., Sabatier,L., Svobodova,M., Beverley,S.M., Spath,G., Brun,R., Pesson,B. & Bulet,P. (2004) Characterization of a defensin from the sand fly Phlebotomus duboscqi induced by challenge with bacteria or the protozoan parasite Leishmania major. Infection And Immunity, 72, 7140-7146.

Hajmova,M., Chang,K.P., Kolli,B. & Volf,P. (2004) Down-regulation of gp63 in Leishmania amazonensis reduces its early development in Lutzomyia longipalpis. Microbes And Infection, 6, 646-649.

Modry,D., Votypka,J. & Svobodova,M. (2004) Note on the taxonomy of Frenkelia microti (Findlay & Middleton, 1934) (Apicomplexa : Sarcocystidae). Systematic Parasitology, 58, 185-187.

Podlipaev,S., Votypka,J., Jirku,M., Svobodova,M. & Lukes,J. (2004) Herpetomonas ztiplika n. sp (Kinetoplastida : Trypanosomatidae): A parasite of the blood-sucking biting midge Culicoides kibunensis Tokunaga, 1937 (Diptera : Ceratopogonidae). Journal Of Parasitology, 90, 342-347.

Soares,R.P.P., Barron,T., McCoy-Simandle,K., Svobodova,M., Warburg,A. & Turco,S.J. (2004) Leishmania tropica: intraspecific polymorphisms in lipophosphoglycan correlate with transmission by different Phlebotomus species. Experimental Parasitology, 107, 105-114.

Svobodova,M., Vorisek,P., Votypka,J. & Weidinger,K. (2004) Heteroxenous coccidia (Apicomplexa : Sarcocystidae) in the populations of their final and intermediate hosts: European buzzard and small mammals. Acta Protozoologica, 43, 251-260.

Volf,P., Hajmova,M., Sadlova,J. & Votypka,J. (2004) Blocked stomodeal valve of the insect vector: similar mechanism of transmission in two trypanosomatid models. International Journal For Parasitology, 34, 1221-1227.

Votypka,J. & Svobodova,M. (2004) Trypanosoma avium: experimental transmission from black flies to canaries. Parasitology Research, 92, 147-151.

Votypka,J., Lukes,J. & Obornik,M. (2004) Phylogenetic relationship of Trypanosoma corvi with other avian trypanosomes. Acta Protozoologica, 43, 225-231.


Rohousova,I., Ozensoy,S., Ozbel,Y. & Volf,P. (2005) Detection of species-specific antibody response of humans and mice bitten by sand flies. Parasitology, 130, 493-499.

Rohousova,I., Volf,P. & Lipoldova,M. (2005) Modulation of murine cellular immune response and cytokine production by salivary gland lysate of three sand fly species. Parasite Immunology, 27, 469-473.

Secundino,N.F.C., Nacif-Pimenta,R., Hajmova,M., Volf,P. & Pimenta,P.F.P. (2005) Midgut muscle network in Lutzomyia longipalpis and Phlebotomus duboscqi sand flies: spatial organization and structural modification after blood meal. Arthropod Structure & Development, 34, 167-178.

Thiakaki,M., Rohousova,I., Volfova,V., Volf,P., Chang,K.P. & Soteriadou,K. (2005) Sand fly specificity of saliva-mediated protective immunity in Leishmania amazonensis-BALB/c mouse model. Microbes And Infection, 7, 760-766.

Dvorak,V., Aytekin,A.M., Alten,B., Skarupova,S., Votypka,J. & Volf,P. (2006) A comparison of the intraspecific variability of Phlebotomus sergenti Parrot, 1917 (Diptera : Psychodidae). Journal Of Vector Ecology, 31, 229-238.

Havelkova,H., Badalova,J., Svobodova,M., Vojtiskova,J., Kurey,I., Vladimirov,V., Demant,P. & Lipoldova,M. (2006) Genetics of susceptibility to leishmaniasis in mice: four novel loci and functional heterogeneity of gene effects. Genes And Immunity, 7, 220-233.

Kato,H., Anderson,J.M., Kamhawi,S., Oliveira,F., Lawyer,P.G., Pham,V.M., Sangare,C.S., Samake,S., Sissoko,I., Garfield,M., Sigutova,L., Volf,P., Doumbia,S. & Valenzuela,J.G. (2006) High degree of conservancy among secreted salivary gland proteins from two geographically distant Phlebotomus duboscqi sandflies populations (Mali and Kenya). Bmc Genomics, 7.

Rohousova,I. & Volf,P. (2006) Sand fly saliva: effects on host immune response and Leishmania transmission. Folia Parasitologica, 53, 161-171.

Sadlova,J., Volf,P., Victoir,K., Dujardin,J.C. & Votypka,J. (2006) Virulent and attenuated lines of Leishmania major: DNA karyotypes and differences in metalloproteinase, GP63. Folia Parasitologica, 53, 81-90.

Svobodova,M., Volf,P. & Votypka,J. (2006) Experimental transmission of Leishmania tropica to hyraxes (Procavia capensis) by the bite of Phlebotomus arabicus. Microbes And Infection, 8, 1691-1694.

Svobodova,M., Votypka,J., Peckova,J., Dvorak,V., Nasereddin,A., Baneth,G., Sztern,J., Kravchenko,V., Orr,A., Meir,D., Schnur,L.F., Volf,P. & Warburg,A. (2006) Distinct transmission cycles of Leishmania tropica in 2 adjacent foci, northern Israel. Emerging Infectious Diseases, 12, 1860-1868.

Benkova,I. & Volf,P. (2007) Effect of temperature on metabolism of Phlebotomus papatasi (Diptera : Psychodidae). Journal Of Medical Entomology, 44, 150-154.

Gomes, RB; Collin, N; Teixeira, C; Jochinn, R; Elnaiem, DE; Volf, P; Costa, C; Valenzuela, JG. 2007. Lutzomyia longipalpis salivary recombinant proteins recognized by human, dog and fox antibodies. AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE 77 (5): 113-113, Suppl. S.


Herder,S., Votypka,J., Jirku,M., Radrova,J., Janzen,C.J. & Lukes,J. (2007) Trypanosoma brucei 29-13 strain is inducible in but not permissive for the tsetse fly vector. Experimental Parasitology, 117, 111-114.

Myskova,J., Svobodova,M., Beverley,S.M. & Volf,P. (2007) A lipophosphoglycan-independent development of Leishmania in permissive sand flies. Microbes And Infection, 9, 317-324.

Sloboda, M; Kamler, M; Bulantova, J; Votypka, J; Modry, D. 2007. A new species of Hepatozoon (Apicomplexa : Adeleorina) from Python regius (Serpentes : Pythonidae) and its experimental transmission by a mosquito vector. JOURNAL OF PARASITOLOGY 93 (5): 1189-1198.


Svobodova,M., Votypka,J., Zidkova,L., Hlavackova,J., Belen,A., Alten,B. & Volf,P. (2007) Phlebotomine sand flies in an endemic focus of cutaneous leishmaniasis in Turkey: identification of the vector. Journal Of Eukaryotic Microbiology, 54, 20S.

Svobodova,M. & Votypka,J. (2007) Occurrence of blood protists in raptors (Falconiformes). Buteo, 10 , 51-56.

Svobodova,M., Zidkova,L., Cepicka,I., Obornik,M., Lukes,J. & Votypka,J. (2007) Sergeia podlipaevi gen. nov., sp nov (Trypanosomatidae, Kinetoplastida), a parasite of biting midges (Ceratopogonidae, Diptera). International Journal Of Systematic And Evolutionary Microbiology, 57, 423-432.

Volf,P. & Myskova,J. (2007) Sand flies and Leishmania: specific versus permissive vectors. Trends in Parasitology, 23, 91-92.

Volf,P., Benkova,I., Myskova,J., Sadlova,J., Campino,L. & Ravel,C. (2007) Increased transmission potential of Leishmania major/Leishmania infantum hybrids. International Journal For Parasitology, 37, 589-593.

Myskova J., Votypka J., Volf P., 2008. Leishmania in sand flies: comparison of quantitative polymerase chain reaction with other techniques to determine the intensity of infection. Journal of Medical Entomology 45: 133-138.


Depaquit J., Lienard E., Verzeaux-Griffon A., Ferte H., Bounamous A., Gantier J.C., Hanafi H.A., Jacobson R.L., Maroli M., Moin-Vaziri V., Muller F., Ozbel Y., Svobodova M., Volf P., Leger N., 2008. Molecular homogeneity in diverse geographical populations of Phlebotomus papatasi (Diptera: Psychodidae) inferred from ND4 mtDNA and ITS2 rDNA: epidemiological consequences. Infection, Genetics and Evolution 8: 159-170.


Rogers M.E., Hajmova M., Joshi M.B., Sadlova J., Dwyer D.M., Volf P., Bates P., 2008. Leishmania chitinase facilitates colonization of sand fly vectors and enhances transmission to mice. Cellular Microbiology 10: 1363-1372.


Hostomska J., Rohousova I., Volfova V., Stanneck D., Mencke N., Volf P., 2008. Kinetics of canine antibody response to saliva of the sand fly Lutzomyia longipalpis. Vector-borne and Zoonotic Diseases 8: 443-450.


Volf P., Hostomska J., Rohousova I., 2008. Molecular crosstalks in Leishmania-sandfly-host relationships. Parasite 15: 237-243.


Volfova V., Hostomska J., Cerny M., Votypka J. and Volf P., 2008. Hyaluronidase of bloodsucking insects and its enhancing effect on Leishmania infection in mice. PLoS Neglected Tropical Diseases 2:e294.


Drahota J., Lipoldova M., Volf P., Rohousova I., 2009. Specificity of anti-saliva immune response in mice repeatedly bitten by Phlebotomus sergenti. Parasite imunology 31: 766-770.


Hostomska J., Volfova V., Mu JB., Garfield M., Rohousova I., Volf P., Valenzuela JG., Jochim RC., 2009. Analysis of salivary transcripts and antigens of the sand fly Phlebotomus arabicus. BMC Genomics 10.


Jecna, L., Svarovska A., Besteiro S., Mottram JC., Coombs GH., Volf P., 2009. Inhibitor of Cysteine Peptidase Does Not Influence the Development of Leishmania mexicana in Lutzomyia longipalpis. Journal Of Medical Entomology 46: 605-609.


Kurey I., Kobets T., Havelkova H., Slapnickova M., Quan L., Trtkova K., Grekov I., Svobodova M., Stassen AP., Hutson A., Demant P., Lipoldova M., 2009. Distinct genetic control of parasite elimination, dissemination, and disease after Leishmania major infection. Immunogenetics 61: 619-633.


Sadlova J., Volf P., 2009. Peritrophic matrix of Phlebotomus duboscqi and its kinetics during Leishmania major development. Cell and Tissue Research 337: 313-325.


Svobodova M., Alten B., Zidkova L., Dvorak V., Hlavackova J., Myskova J., Seblova V., Kasap OE., Belen A., Votypka J., Volf P., 2009. Cutaneous leishmaniasis caused by Leishmania infantum transmitted by Phlebotomus tobbi. International Journal for Parasitology 39: 251-253.


Votypka J., Lantova L., Ghosh K., Braig H., Volf P., 2009. Molecular Characterization of Gregarines from Sand Flies (Diptera: Psychodidae) and Description of Psychodiella n. g. (Apicomplexa: Gregarinida). The Journal of eukaryotic microbiology 56: 583-588.


Votypka J., Synek P., Svobodova M., 2009. Endophagy of biting midges attacking cavity-nesting birds. Medical and Veterinary Entomology 23: 277-280.


Volf P., Sadlova J., 2009. Sex in Leishmania. Science 324: 1644-1644.


Clements M.F., Gidwani K., Kumar R., Hostomská J., Dinesh D.S., Kumar V., Das P., Muller I., Hamilton G., Volfova V., Boleart M., Das M., Rijal S., Picado A., Volf P., Sundar S., Davies C.R., Rogers M.E. (2010): Measurement of recent exposure to Phlebotomus argentipes, the vector of indian visceral leishmaniasis, by using human antibody responses to sand fly saliva. Am. J. Trop. Med. Hyg. 82: 801-807.


Kobets T, Badalová J, Grekov I, Havelková H, Svobodová M, Lipoldová M.

Leishmania parasite detection and quantification using PCR-ELISA. Nat Protoc.

2010;5(6):1074-80. Epub 2010 May 20. PubMed PMID: 20539283.


Lantova L., Ghosh K., Svobodova M., Braig H.R., Rowton E., Weina P., Volf P. , Votypka J. (2010). The life cycle and host specificity of Psychodiella sergenti n.sp. and Ps. tobbi n. sp. (Protozoa: Apicomplexa) in sand flies Phlebotomus sergenti and Ph. tobbi (Diptera: Psychodidae). Journal of Invertebrate Pathology 105: 182-189.


Sadlova J., Price H.P., Smith B.A., Votypka J., Volf P., Smith D.F. (2010): The stage-regulated HASPB and SHERP proteins are essential for differentiation of the protozoan parasite Leishmania major in its sand fly vector, Phlebotomus papatasi. Cellular Microbiology 12: 1765-1779.


Svárovská A., Ant T.H., Seblova V., Jecna L., Beverley S.M., Volf. P. (2010): Leishmaia major glycosylation mutants require phosphoglycans (lpg2-) but not lipophosphoglycan (lpg1-) for survival in permissive sand fly vectors. PLoS Neglected Tropical Diseases 4: e580.


Talmi-Frank D, Jaffe CL, Nasereddin A, Warburg A, King R, Svobodova M, Peleg

O, Baneth G. Leishmania tropica in rock hyraxes (Procavia capensis) in a focus of

human cutaneous leishmaniasis. Am J Trop Med Hyg. 2010 May;82(5):814-8.


Teixeira C., Gomes R. Collin N., Reynoso D., Jochim R., Oliveira F., Seitz A., Elnaiem D., Caldas A., de Souza A.P., Brodskyn C.I., de Oliveira C.I., Mendonca I., Costa C.H.N., Volf P., Barral A., Kamhawi S., Valenzuela J.G. (2010): Discovery of markers of exposure specific to bites of Lutzomyia longipalpis, the vector of Leishmania infantum chagasi in Latin America. PLoS Neglected Tropical Diseases 4: e638.


Vinkler M., Schnitzer J., Munclinger P., Votýpka J. & Albrecht T. (2010) Haematological health assessment in a passerine with extremely high proportion of basophils in peripheral blood. Journal of Ornithology 151: 841–849.


Votýpka J., Maslov A., Yurchenko V., Jirků M., Kment P., Lun Z.-R. & Lukeš J. (2010) Probing into the diversity of trypanosomatid flagellates parasitizing insect hosts in South-West China reveals both endemism and global dispersal. Molecular Phylogenetics and Evolution 54: 243253.


Wilson R., Bates M.D., Dostalova A., Jecna L., Dillon R.J., Volf P., Bates P.A. (2010): Stage-specific adhesion of Leishmania promastigotes to sand fly midgut assessed using an improved comparative binding assay. PLoS Neglected Tropical Diseases 4: e 816.


Zídková L., Čepička I., Votýpka J. & Svobodová M. (2010) Herpetomonas trimorpha sp. nov. (Trypanosomatidae, Kinetoplastida), a parasite of the biting midge Culicoides truncorum (Ceratopogonidae, Diptera). International Journal of Systematic and Evolutionary Microbiology 60: 22362246.


Dvorak, V., Votypka, J., Aytekin, A. M., Alten, B.,, Volf, P. (2011) Intraspecific variability of natural populations of Phlebotomus sergenti, the main vector of Leishmania tropica. Journal of Vector Ecology 36: S49-S57.


Rohousova, I., Hostomska, J., Vlkova, M., Kobets, T., Lipoldova, M., Volf, P. (2011) The protective effect against Leishmania infection conferred by sand fly bites is limited to short-term exposure. International Journal for Parasitology 41: 481-485.


Volf, P., Volfova, V. (2011) Establishment and maintanence of sand fly colonies. Journal of Vector Ecology 36: S1-S9.

Stage-Specific Adhesion of Leishmania Promastigotes to Sand Fly Midguts Assessed Using an Improved Comparative Binding Assay


The binding of Leishmania promastigotes to the midgut epithelium is regarded as an essential part of the life-cycle in the sand fly vector, enabling the parasites to persist beyond the initial blood meal phase and establish the infection. However, the precise nature of the promastigote stage(s) that mediate binding is not fully understood.

Subscribe to RSS - Charles University in Prague (CUNI)